The immunizing potential of sporulated oocysts of Eimeria nieschulzi exposed to heat and Co-60 gamma-radiation.

On the status of Eimeria nieschulzi oocysts embedded in resin eleven years ago: a permanent method for preserving coccidian oocysts.

Sporulated oocysts of Eimeria nieschulzi that were fixed and mounted on glass slides in polymerized resin in 1976 are examined. Size, shape, and integrity of oocysts and sporocysts are compared to similar observations we made in 1977 and reported in 1978 (Journal of Parasitology 64: 163-164). Our conclusion is that the methods we reported on in 1978 provide one opportunity to produce permanent ...

Fixing coccidian oocysts is not an adequate solution to the problem of preserving protozoan type material.

Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K2Cr2O7. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups ...

Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells

The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research. Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species. For several mammalian Eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts...

Effects of essential oils combination on sporulation of turkey (Meleagris gallopavo) Eimeria oocysts

Avian coccidiosis is the most important parasitic disease in poultry production, which inflicts numerous losses to the industry. The extensive use of anticoccidial drugs leads to parasite resistance and drug residue in poultry products. In the present study, we aimed to investigate the effects of three famous essential oils (EOs) and their combination on inactivation of mixed oocysts of Eimeria...

Permanent light microscopy slides of Eimeria nieschulzi oocysts.